High-fidelity amplification
High-fidelity DNA polymerases provide exceptional accuracy, speed and performance for all PCR applications.
These enzymes are significantly superior to Pfu and Taq polymerases in the following parameters:
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> processivity
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> synthesis accuracy
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> fragment amplification rate
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> resistance to PCR inhibitors
Comparison of synthesis of long fragments:
Analysis of salt resistance of Pfu DNA polymerase and Fusion DNA polymerase using the example of fragment amplification in the presence of KCl:
Name
Catalog #
Qty
Name
Fusion 2.0 polymerase
A modified version of Fusion DNA polymerase obtained by merging the thermostable DNA polymerase of Pyrococcus furiosus (Pfu) and the DNA-binding protein of the thermophilic archaea species Saccharolobus solfataricus (Sso7d). A number of mutations were added to Fusion 2.0 polymerase, increasing the accuracy of the enzyme by approximately 3 times or ~15 times relative to the “native” Taq DNA polymerase
Name
PCR amplification kit with Fusion DNA polymerase
A set of reagents for performing PCR with high-fidelity DNA polymerase. The kit contains individual components such as magnesium ions, a mixture of deoxynucleotide triphosphates (dNTPs) and dimethyl sulfoxide, which allows you to optimize the amplification conditions for the experimenter’s tasks.
Name
Fusion DNA polymerase (Pfu-Sso7d)
Fusion DNA polymerase consists of Pyrococcus furiosus (Pfu) thermostable DNA polymerase and thermophilic archaea DNA-binding protein Sulfolobus solfataricus (Sso7d). The Sso7d protein binds to the minor groove of double-stranded DNA and stabilizes the polymerase-template complex. Due to this, Fusion DNA polymerase has increased processivity, synthesis accuracy, amplification rate, and resistance to PCR inhibitors.