BioMaster UDG HS-qPCR SYBR Blue (2×)

2× reaction mix containing Hot-Start Taq DNA polymerase, dUTP and N-uracil-DNA glycosylase for real-time PCR with SYBR Green I.
  • Hot-start real-time PCR with oligonucleotide probes
  • Conventional PCR
  • High-throughput PCR
  • Genotyping

Basic description

BioMaster UDG HS-qPCR SYBR Blue (2×) contains 2× BioMaster UDG HS-qPCR SYBR Blue reaction mix, 50 mM MgCl2 solution and sterile water. The master mix is developed for quantitative real-time PCR with fluorescent dye SYBR Green I. BioMaster UDG HS-qPCR SYBR Blue (2×) includes all components (except for DNA template and primers) necessary to perform PCR: highly processive recombinant HS-Taq DNA polymerase, N-uracil-DNA glycosylase, dNTP mix, PCR buffer, Mg2+, SYBR Green I and inert dye.

The mix is optimized for conducting efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples. The solution includes substances that increase half-life and processivity of HS-Taq DNA polymerase by enhancing its stability during PCR. BioMaster UDG HS-qPCR SYBR Blue (2×) contains components that influence primer annealing temperature and characteristics of template melting thus enabling to increase the specificity of PCR and use templates with complicated spatial structure.

N-uracil-DNA glycosylase (UDG) and dUTP (in proportion with dTTP) provide protection from amplicon transfer between reaction solutions (cross-contamination). DNA polymerase included in the mix is inactive at room temperature; its activation requires preheating at 95 °C for 5 min. Use of the kit saves time and minimizes contamination risk due to reduced number of pipetting steps.

Blue hue of the reaction solution provided by the inert dye allows control when using multi-well plates. Use of the kit saves time and minimizes contamination risk due to reduced number of pipetting steps. Low concentration of Mg2+ in the reaction solution and additional tube with MgCl2 allow optimizing reaction conditions for individual primer/template system.

Areas of use

  • Classic PCR with "hot" start
  • Genotyping

Reaction mixture properties

  • The reaction mix is inactive at room temperature due to the Hot-Start technology and is activated after incubation at 95 °C for 5 min
  • The presence of dUTP ensures incorporation of uridine into each synthesized DNA strand, UDG is capable of eliminating uracil from single- and double-stranded DNA molecules
  • The mix is optimized for the specific performance of HS-Taq DNA polymerase, long-term storage (the storage of BioMaster UDG HS-qPCR SYBR Blue (2×) at room temperature for 7 days does not affect PCR efficiency), multiple thawing-freezing cycles

Benefits of using

  • Hot-start enzyme increases reaction specificity and sensitivity
  • Activation of HS-Taq DNA polymerase takes not more than 5 min
  • High selectivity and reaction yield
  • Reduced preparation time
  • Prevents re-amplification of PCR carryover products
  • Standardized conditions of the same-type reactions (reduced pipetting error during mixing PCR components from experiment to experiment)
  • Minimized efforts


1 year (under proper storage and transportation conditions).


at -20 °С; not more than 50 thawing-freezing cycles.

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General documentation