BioMaster UDG HS-qPCR SYBR Blue (2×)
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Features
2× reaction mix containing Hot-Start Taq DNA polymerase, dUTP and N-uracil-DNA glycosylase for real-time PCR with SYBR Green I.
Application
Hot-start real-time PCR with oligonucleotide probes
Conventional PCR
High-throughput PCR
Genotyping
MНC031-400
Number of reactions (25 μl)
400
€70
MНC031-2040
Number of reactions (25 μl)
2040
€340
MНC031-2040
Number of reactions (25 μl)
2040
€2,720
Basic description
BioMaster UDG HS-qPCR SYBR Blue (2×) contains 2× BioMaster UDG HS-qPCR SYBR Blue reaction mix, 50 mM MgCl2 solution and sterile water. The master mix is developed for quantitative real-time PCR with fluorescent dye SYBR Green I. BioMaster UDG HS-qPCR SYBR Blue (2×) includes all components (except for DNA template and primers) necessary to perform PCR: highly processive recombinant HS-Taq DNA polymerase, N-uracil-DNA glycosylase, dNTP mix, PCR buffer, Mg2+, SYBR Green I and inert dye.
The mix is optimized for conducting efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples. The solution includes substances that increase half-life and processivity of HS-Taq DNA polymerase by enhancing its stability during PCR. BioMaster UDG HS-qPCR SYBR Blue (2×) contains components that influence primer annealing temperature and characteristics of template melting thus enabling to increase the specificity of PCR and use templates with complicated spatial structure.
N-uracil-DNA glycosylase (UDG) and dUTP (in proportion with dTTP) provide protection from amplicon transfer between reaction solutions (cross-contamination). DNA polymerase included in the mix is inactive at room temperature; its activation requires preheating at 95 °C for 5 min. Use of the kit saves time and minimizes contamination risk due to reduced number of pipetting steps.
Blue hue of the reaction solution provided by the inert dye allows
control when using multi-well plates. Use of the kit saves time and
minimizes contamination risk due to reduced number of pipetting steps.
Low concentration of Mg2+ in the reaction solution and additional tube
with MgCl2 allow optimizing reaction conditions for individual
primer/template system.
Areas of use
Classic PCR with "hot" start Genotyping Reaction mixture properties
The reaction mix is inactive at room temperature due to the Hot-Start technology and is activated after incubation at 95 °C for 5 min
The presence of dUTP ensures incorporation of uridine into each synthesized DNA strand, UDG is capable of eliminating uracil from single- and double-stranded DNA molecules
The mix is optimized for the specific performance of HS-Taq DNA polymerase, long-term storage (the storage of BioMaster UDG HS-qPCR SYBR Blue (2×) at room temperature for 7 days does not affect PCR efficiency), multiple thawing-freezing cycles
Benefits of using
Hot-start enzyme increases reaction specificity and sensitivity
Activation of HS-Taq DNA polymerase takes not more than 5 min
High selectivity and reaction yield
Reduced preparation time
Prevents re-amplification of PCR carryover products
Standardized conditions of the same-type reactions (reduced pipetting error during mixing PCR components from experiment to experiment)
Minimized efforts
Storage
1 year (under proper storage and transportation conditions).
Transportation
at -20 °С; not more than 50 thawing-freezing cycles.
Product passport
We carefully prepare our products and are ready to confirm their suitability with a passport.
Each box of our products has a unique lot number in the AA-000-0000 format.
