BioMaster UDG HS-qPCR Lo-ROX SYBR (2×) contains 2× BioMaster UDG HS-qPCR Lo-ROX SYBR reaction mix, 50 mM MgCl2 solution and sterile water. The mix is developed for quantitative real-time PCR with fluorescent dye SYBR Green I on PCR platforms that utilize ROX as a reference dye. BioMaster UDG HS-qPCR Lo-ROX SYBR (2×) includes all components (except for DNA template and primers) necessary for PCR: highly processive recombinant HS-Taq DNA polymerase, N-uracil-DNA glycosylase, dNTP mix, PCR buffer, Mg2+ solution, SYBR Green I, fluorescent dye ROX, and inert dye.
BioMaster UDG HS-qPCR Lo-ROX SYBR (2×) is optimized for efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples. BioMaster UDG HS-qPCR Lo-ROX SYBR (2×) mix contains substances influencing primer annealing temperature and characteristics of template melting thus enabling to increase PCR specificity and use templates with complicated spatial structure.
N-uracil-DNA glycosylase (UDG) and dUTP (in proportion with dTTP)
provide protection from amplicon transfer between reaction solutions
(cross-contamination). DNA polymerase included in the mix is inactive at
room temperature, its activation requires preheating at 95 °C for 5
min. Blue hue of the reaction solution provided by the inert dye allows
control when using multi-well plates. The master mix is ideally suitable
for PCR platforms that use ROX passive dye as a reference guide, such
as Life Technologies (ABI) 7500, 7500 Fast, ViiA 7, QuantStudio 12K;
Stratagene Mx4000, Mx3005P, and Mx3000P.