BioMaster HS-qPCR SYBR Blue(2×)

2× reaction mix containing Hot-Start Taq DNA polymerase for real-time PCR with SYBR Green I.
  • Real-time PCR with intercalating dye SYBR Green I
  • Conventional PCR

Basic description

BioMaster HS-qPCR SYBR Blue (2×) contains 2× BioMaster HS-qPCR SYBR Blue reaction mix and sterile water. The master mix is developed for quantitative real-time PCR with fluorescent dye SYBR Green I. BioMaster HS-qPCR SYBR Blue (2×) contains every component (except for DNA template and primers) necessary for PCR: HS-Taq DNA polymerase, dNTP mix, PCR buffer, Mg2+, SYBR Green I and inert dye.

The mix is optimized for conducting efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples. The solution contains substances that increase half-life and processivity of HS-Taq DNA polymerase by enhancing its stability during PCR. BioMaster HS-qPCR SYBR Blue (2×) includes components that influence primer annealing temperature and characteristics of template melting thus enabling to increase the specificity of PCR and use templates with complicated spatial structure. Blue hue of the reaction solution provided by the inert dye allows control when using multi-well plates. Use of the kit saves time and minimizes contamination risk due to reduced number of pipetting steps.

Areas of use

  • Classic PCR with "hot" start

Reaction mixture properties

  • optimized for real-time PCR
  • contains substances that allow long-term storage (the storage of BioMaster HS-qPCR SYBR Blue (2×) at room temperature for one month does not affect the efficiency of PCR) and multiple thawing-freezing cycles

Benefits of using

  • The mix is colored for easy pipetting
  • Reduced preparation time
  • Low chance of contamination while preparing PCR solution
  • Standardized conditions of the same-type reactions (reduced pipetting error during mixing PCR components from experiment to experiment)
  • Minimized efforts


1 year (under proper storage and transportation conditions).


at -20 °С; not more than 50 thawing-freezing cycles.

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