BioMaster HS-qPCR Hi-ROX SYBR (2×)

2× reaction mix containing Hot-Start Taq DNA polymerase for real-time PCR with SYBR Green I; contains ROX at high concentration for studies involving use of specific equipment.
  • Real-time PCR with intercalating dye SYBR Green I
  • Conventional PCR
  • High-throughput PCR
  • Genotyping

Basic description

BioMaster HS-qPCR Hi-ROX SYBR (2×) contains 2× BioMaster HS-qPCR Hi-ROX SYBR reaction mix, 50 mM MgCl2 solution and sterile water. The mix is developed for quantitative real-time PCR with the use of fluorescent dye SYBR Green I on PCR platforms that utilize ROX as a reference dye. BioMaster HS-qPCR Hi-ROX SYBR (2×) includes all the components (except for DNA template and primers) necessary for PCR: highly processive recombinant HS-Taq DNA polymerase, dNTP mix, PCR buffer, Mg2+ solution, SYBR Green I, fluorescent dye ROX, and inert dye.

BioMaster HS-qPCR Hi-ROX SYBR (2×) is optimized for efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples. BioMaster HS-qPCR Hi-ROX SYBR (2×) includes additives that influence primer annealing temperature and characteristics of template melting thus enabling to increase PCR specificity and use templates with complicated spatial structure.

DNA polymerase included in the mix is inactive at room temperature and its activation requires preheating at 95 °C for 5 min. Blue hue of the reaction solution provided by the inert dye allows control when using multi-well plates. Use of the mix saves time and minimizes contamination risk due to reduced number of pipetting steps. Low concentration of Mg2+ in the reaction solution and additional tube with 50 mM MgCl2 solution allow optimization of reaction conditions for individual primer/template system.

The master mix is ideally suitable for PCR platforms that use ROX passive dye as a reference guide, such as Life Technologies (ABI) 7000, 7300, 7700, 7900, 7900HT, and StepOnePlus.

Areas of use

  • Classic PCR with "hot" start
  • Genotyping

Reaction mixture properties

  • The mix is inactive at room temperature due to the Hot-Start technology and is activated after incubation at 95 °C for 5 min
  • Allows normalization using ROX dye as a reference
  • The mix is optimized for the specific performance of HS-Taq DNA polymerase, long-term storage (the storage of BioMaster HS-qPCR Hi-ROX SYBR (2×) at room temperature for a month does not affect PCR efficiency), multiple thawing-freezing cycles

Benefits of using

  • Enzyme with hot-start capability increases specificity, sensitivity and reaction yield
  • HS-Taq DNA polymerase activation requires not more than 5 min heating
  • The mix is colored for easy pipetting
  • Reduced preparation time
  • Low contamination risk during preparation of PCR solution
  • Allows data normalization
  • Standardized conditions of the same-type reactions (reduced pipetting error during mixing PCR components from experiment to experiment)


1 year (under proper storage and transportation conditions).


at -20 °С; not more than 50 thawing-freezing cycles.

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