BioMaster UDG HS-qPCR (2×)

2× reaction mix containing Hot-Start Taq DNA polymerase, dUTP and N-uracil-DNA glycosylase for real-time PCR with fluorescent probes.
  • Hot-start real-time PCR with oligonucleotide probes
  • Conventional PCR
  • High-throughput PCR
  • Multiplex PCR
  • Genotyping

Basic description

BioMaster UDG HS-qPCR (2×) contains 2× BioMaster UDG HS-qPCR reaction mix and sterile water. The master mix is developed for quantitative real-time PCR with fluorescently labeled probes. BioMaster UDG HS-qPCR (2×) includes all components (except for DNA template, primers and probe) necessary to perform PCR: HS-Taq DNA polymerase, N-uracil-DNA glycosylase, dNTP mix, PCR buffer, and Mg2+.

The mix is optimized for conducting efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples. The solution includes substances that increase half-life and processivity of HS-Taq DNA polymerase by enhancing its stability during PCR. BioMaster UDG HS-qPCR (2×) does not contain components that influence primer annealing temperature and characteristics of template melting.

N-uracil-DNA glycosylase (UDG) and dUTP (in proportion with dTTP) provide protection from amplicon transfer between reaction solutions (cross-contamination). DNA polymerase included in the mix is inactive at room temperature; its activation requires preheating at 95 °C for 5 min. Use of the kit saves time and minimizes contamination risk due to reduced number of pipetting steps.

Areas of use

  • Classic PCR with "hot" start
  • Genotyping
  • PCR in real time with "hot" start, with probes and intercalating dyes
  • PCR multiplex

Reaction mixture properties

  • The reaction mix is inactive at room temperature due to the Hot-Start technology and is activated after incubation at 95 °C for 5 min
  • The presence of dUTP ensures incorporation of uridine into each synthesized DNA strand, UDG is capable of eliminating uracil from single- and double-stranded DNA molecules
  • The mix is optimized for the specific performance of HS-Taq DNA polymerase, long-term storage (the storage of BioMaster UDG HS-qPCR (2×) at room temperature for 7 days does not affect PCR efficiency), multiple thawing-freezing cycles

Benefits of using

  • Hot-start enzyme increases reaction specificity and sensitivity
  • Activation of HS-Taq DNA polymerase takes not more than 5 min
  • High selectivity and reaction yield
  • Reduced preparation time
  • Prevents re-amplification of PCR carryover products
  • Standardized conditions of the same-type reactions (reduced pipetting error during mixing PCR components from experiment to experiment)
  • Minimized efforts


1 year (under proper storage and transportation conditions).


at -20 °С; not more than 50 thawing-freezing cycles.

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General documentation