BioMaster UDG HS-qPCR (2×) contains 2× BioMaster UDG HS-qPCR reaction mix and sterile water. The master mix is developed for quantitative real-time PCR with fluorescently labeled probes. BioMaster UDG HS-qPCR (2×) includes all components (except for DNA template, primers and probe) necessary to perform PCR: HS-Taq DNA polymerase, N-uracil-DNA glycosylase, dNTP mix, PCR buffer, and Mg2+.
The mix is optimized for conducting efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples. The solution includes substances that increase half-life and processivity of HS-Taq DNA polymerase by enhancing its stability during PCR. BioMaster UDG HS-qPCR (2×) does not contain components that influence primer annealing temperature and characteristics of template melting.
N-uracil-DNA glycosylase (UDG) and dUTP (in proportion with dTTP) provide protection from amplicon transfer between reaction solutions (cross-contamination). DNA polymerase included in the mix is inactive at room temperature; its activation requires preheating at 95 °C for 5 min. Use of the kit saves time and minimizes contamination risk due to reduced number of pipetting steps.
Areas of use
Classic PCR with "hot" start
PCR in real time with "hot" start, with probes and intercalating dyes
Reaction mixture properties
The reaction mix is inactive at room temperature due to the Hot-Start technology and is activated after incubation at 95 °C for 5 min
The presence of dUTP ensures incorporation of uridine into each synthesized DNA strand, UDG is capable of eliminating uracil from single- and double-stranded DNA molecules
The mix is optimized for the specific performance of HS-Taq DNA polymerase, long-term storage (the storage of BioMaster UDG HS-qPCR (2×) at room temperature for 7 days does not affect PCR efficiency), multiple thawing-freezing cycles
Benefits of using
Hot-start enzyme increases reaction specificity and sensitivity
Activation of HS-Taq DNA polymerase takes not more than 5 min
High selectivity and reaction yield
Reduced preparation time
Prevents re-amplification of PCR carryover products
Standardized conditions of the same-type reactions (reduced pipetting error during mixing PCR components from experiment to experiment)
1 year (under proper storage and transportation conditions).
at -20 °С; not more than 50 thawing-freezing cycles.
We carefully prepare our products and are ready to confirm their suitability with a passport.
Each box of our products has a unique lot number in the AA-000-0000 format.
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