BioMaster HS-qPCR (2×) contains 2× BioMaster HS-qPCR reaction mix and sterile water. The master mix is developed for quantitative real-time PCR with fluorescently labeled probes. BioMaster HS-qPCR (2×) includes all components (except for DNA template, primers and probe) necessary to perform PCR: HS-Taq DNA polymerase, dNTP mix, 2× PCR buffer, and Mg2+.
The mix is optimized for conducting efficient and reproducible hot-start
real-time PCR of genomic, plasmid and viral DNA samples. The solution
contains substances that increase half-life and processivity of HS-Taq DNA polymerase by enhancing its stability during PCR. BioMaster HS-qPCR (2×)
includes components that influence primer annealing temperature and
characteristics of template melting thus enabling to increase PCR
specificity and use templates with complicated spatial structure. DNA
polymerase included in the mix is inactive at room temperature; its
activation requires preheating at 95 °C for 5 min. Use of the kit saves
time and minimizes contamination risk due to reduced number of pipetting
Areas of use
Classic PCR with "hot" start
PCR in real time with "hot" start, with probes and intercalating dyes
Reaction mixture properties
The reaction mix is optimized for hot-start real-time PCR with fluorescently labeled probes
The mix contains substances increasing storage terms (the storage of BioMaster HS-qPCR (2×) at room temperature for one month does not affect PCR efficiency) and allowing multiple thawing-freezing cycles
Benefits of using
Hot-start enzyme increases specificity, sensitivity and reaction yield
Activation of HS-Taq DNA polymerase takes not more than 5 min
Reduced preparation time
Low chance of contamination during preparation of PCR solution
Standardized conditions of the same-type reactions (reduced pipetting error during mixing PCR components from experiment to experiment)
1 year (under proper storage and transportation conditions).
at -20 °С; not more than 50 thawing-freezing cycles.
We carefully prepare our products and are ready to confirm their suitability with a passport.
Each box of our products has a unique lot number in the AA-000-0000 format.
Enter it in the box below and download the certificate in PDF format.