BioMaster HS-qPCR (2×)

2× reaction mix containing Hot-Start Taq DNA polymerase for real-time PCR with fluorescent probes.
  • Hot-start real-time PCR with oligonucleotide probes
  • Conventional PCR
  • High-throughput PCR
  • Multiplex PCR
  • Genotyping

Basic description

BioMaster HS-qPCR (2×) contains 2× BioMaster HS-qPCR reaction mix and sterile water. The master mix is developed for quantitative real-time PCR with fluorescently labeled probes. BioMaster HS-qPCR (2×) includes all components (except for DNA template, primers and probe) necessary to perform PCR: HS-Taq DNA polymerase, dNTP mix, 2× PCR buffer, and Mg2+.

The mix is optimized for conducting efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples. The solution contains substances that increase half-life and processivity of HS-Taq DNA polymerase by enhancing its stability during PCR. BioMaster HS-qPCR (2×) includes components that influence primer annealing temperature and characteristics of template melting thus enabling to increase PCR specificity and use templates with complicated spatial structure. DNA polymerase included in the mix is inactive at room temperature; its activation requires preheating at 95 °C for 5 min. Use of the kit saves time and minimizes contamination risk due to reduced number of pipetting steps.

Areas of use

  • Classic PCR with "hot" start
  • Genotyping
  • PCR in real time with "hot" start, with probes and intercalating dyes
  • PCR multiplex

Reaction mixture properties

  • The reaction mix is optimized for hot-start real-time PCR with fluorescently labeled probes
  • The mix contains substances increasing storage terms (the storage of BioMaster HS-qPCR (2×) at room temperature for one month does not affect PCR efficiency) and allowing multiple thawing-freezing cycles

Benefits of using

  • Hot-start enzyme increases specificity, sensitivity and reaction yield
  • Activation of HS-Taq DNA polymerase takes not more than 5 min
  • Reduced preparation time
  • Low chance of contamination during preparation of PCR solution
  • Standardized conditions of the same-type reactions (reduced pipetting error during mixing PCR components from experiment to experiment)
  • Minimized efforts


1 year (under proper storage and transportation conditions).


at -20 °С; not more than 50 thawing-freezing cycles.

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