BioMaster HS-Taq PCR Kit (+MgCl2) contains recombinant HS-Taq DNA polymerase and solutions of every component needed for performing conventional PCR under hot-start conditions, except for DNA template and primers. The mix includes: HS-Taq DNA polymerase solution (5 U/µl), 5× PCR buffer (+MgCl2), 50 mM MgCl2, 50× dNTP mix and 6× loading buffer. All reagents are of high quality and optimized for PCR.
HS-Taq DNA polymerase is a recombinant Taq DNA polymerase inactivated by specific monoclonal antibodies. HS-Taq DNA polymerase remains inactive at temperatures lower than 70 °С, which allows to avoid the formation of non-specific products and primer dimers at low temperatures during preliminary steps. The activation of HS-Taq DNA polymerase is achieved during the first cycle of PCR amplification after short incubation at 95 °С for 5 min. Recombinant Taq DNA polymerase has 5´-3´ DNA-dependent DNA polymerase activity and 5´-3´ exonuclease activity of native Taq DNA polymerase from Thermus aquaticus. The extension rate of Taq DNA polymerase depends on the complexity of DNA template and is approximately 1 kbp/min. Recombinant Taq DNA polymerase is ideal for conventional PCR of templates up to 5 kbp.
5× PCR buffer (+MgCl2) is optimized for performing efficient and reproducible PCR. The buffer contains MgCl2 (10 mM) and components increasing half-life and processivity of HS-Taq
DNA polymerase by enhancing its stability during PCR. The buffer is
chemically stable, inert and does not interfere with optimal annealing
temperature or the parameters of template melting. Additional solutions
of 50 mM MgCl2 and 50× dNTP mix included in the kit allow optimizing PCR
conditions for individual primer pairs, while 6× loading buffer eases
sample preparation for analysis and allows monitoring the progress of
Areas of use
Classic PCR with "hot" start
Development of PCR products for TA cloning
RT-PCR one- and two-step
Benefits of using
Enzyme with hot-start capability increases specificity and sensitivity of PCR
HS-Taq DNA polymerase activation requires 5 min heating
Increased selectivity and reaction yield
PCR products can be further subjected to TA cloning due to the presence of deoxyadenosine overhangs in amplified DNA
1 year (under proper storage and transportation conditions).
at -20 °С; not more than 50 thawing-freezing cycles.
We carefully prepare our products and are ready to confirm their suitability with a passport.
Each box of our products has a unique lot number in the AA-000-0000 format.
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