BioMaster HS-Taq PCR-Spec (2 ×) is designed for PCR of DNA templates with a complex spatial structure or with GC-rich regions. Kit BioMaster HS-Taq PCR-Spec (2 ×) contains 2 × reaction mixture BioMaster HS-Taq PCR-Spec (2 ×), 50 mM MgCl2 and sterile water. The BioMaster HS-Taq PCR-Spec (2 ×) reaction mixture contains all the necessary components for PCR (excluding the DNA template and primers) highly-processed recombinant HS-Taq DNA polymerase with a “hot” start, a mixture of deoxynucleoside triphosphates, 2 × PCR -buffer, Mg2 +.
The mixture is optimized for efficient and reproducible hot start PCR. The mixture contains additives that increase the half-life and processivity of HS-Taq DNA polymerase by increasing its stability during PCR. BioMaster HS-Taq PCR-Spec (2 ×) the reaction mixture is chemically stable, inert and does not change the optimal annealing temperature of the primers or the melting characteristics of the matrix. Its DNA polymerase is inactive at room temperature. To activate it, the reaction mixture must be heated at 95 ° C for 5 min. The MgCl2 solution included in the kit makes it easy to optimize the reaction mixture for a specific template-primer system. The presented form of the PCR kit saves time and reduces the likelihood of contamination due to the small number of pipetting steps.
Areas of use
- Classic PCR with "hot" start
- Development of PCR products for TA cloning
- PCR complex and GC-rich regions
- RT-PCR one- and two-step
Reaction mixture properties
The mixture is optimized for the specific work of HS-Taq DNA polymerase, long-term storage (BioMaster HS-Taq PCR-Spec (2 ×) PCR for 30 days at room temperature does not reduce the efficiency of PCR), repeated freezing-thawing;
in a place protected from light: at +25 ° С - 7 days; at +4 ° С - 6 months; at -20 ° С - 1 year; no more than 50 freeze-thaw cycles.
at 0 - +4 ° С, transportation at room temperature up to 3 days is allowed.