BioMaster HS-Taq PCR-Sp (2×)
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Features
The mixture is optimized for efficient and reproducible hot-start PCR on complex-structured and / or GC-rich DNA templates.
MH011-200
Number of reactions (50 μl)
200
€42
MH011-1020
Number of reactions (50 μl)
1020
€200
MH011-1020
Number of reactions (50 μl)
1020
€1,600
Basic description
BioMaster HS-Taq PCR-Spec (2 ×) is designed for PCR of DNA templates with a complex spatial structure or with GC-rich regions. Kit BioMaster HS-Taq PCR-Spec (2 ×) contains 2 × reaction mixture BioMaster HS-Taq PCR-Spec (2 ×), 50 mM MgCl2 and sterile water. The BioMaster HS-Taq PCR-Spec (2 ×) reaction mixture contains all the necessary components for PCR (excluding the DNA template and primers) highly-processed recombinant HS-Taq DNA polymerase with a “hot” start, a mixture of deoxynucleoside triphosphates, 2 × PCR -buffer, Mg2 +.
Areas of use
Classic PCR with "hot" start Development of PCR products for TA cloning PCR complex and GC-rich regions RT-PCR one- and two-step Reaction mixture properties
The mixture is optimized for the specific work of HS-Taq DNA polymerase, long-term storage (BioMaster HS-Taq PCR-Spec (2 ×) PCR for 30 days at room temperature does not reduce the efficiency of PCR), repeated freezing-thawing;
Benefits of using
Amplifies matrices with a GC content of more than 66%
The hot start enzyme increases the specificity, sensitivity and yield of the reaction;
It takes no more than 5 minutes to activate HS-Taq DNA polymerase;
The time for preparing the reaction is reduced;
Reduces the likelihood of contamination when mixing PCR components;
The conditions for staging the same type of reactions are standardized (the error decreases when mixing PCR components in different experiments);
Possibility of TA cloning of PCR products due to deoxyadenosine residues protruding at the ends of the amplified DNA fragments.
Storage
in a place protected from light: at +25 ° С - 7 days; at +4 ° С - 6 months; at -20 ° С - 1 year; no more than 50 freeze-thaw cycles.
Transportation
at 0 - +4 ° С, transportation at room temperature up to 3 days is allowed.
Product passport
We carefully prepare our products and are ready to confirm their suitability with a passport.
Each box of our products has a unique lot number in the AA-000-0000 format.
