BioMaster HS-Taq PCR-Color (2×) kit contains 2× BioMaster HS-Taq PCR-Color reaction mix, 50 mM MgCl2 and sterile water. BioMaster HS-Taq PCR-Color (2×) reaction mix has been developed for \ PCR analysis of a large number of samples. BioMaster HS-Taq PCR-Color (2×) reaction mix includes all of the components (except for DNA template and primers) necessary to carry out PCR: highly processive recombinant HS-Taq DNA polymerase, deoxynucleoside triphosphate mixture, 2× PCR buffer, Mg2+ and marker dyes.
The mix is optimized for efficient and reproducible hot-start PCR. The master mix is supplemented with additives that increase the half-life and processivity of HS-Taq DNA polymerase by enhancing its stability during PCR. BioMaster HS-Taq PCR-Color (2×) reaction mix is chemically stable, inert and does not interfere with optimal annealing temperature or the parameters of template melting. The presented DNA polymerase is inactive at room temperature; preheating of reaction solution at 95 °C for 5 min is required for enzyme activation. Additional solution of MgCl2 allows easy optimization for each individual primer/template system. Use of the kit saves time and minimizes contamination risk due to reduced number of pipetting steps. The included dyes and increased density of the solution allow loading the reaction mix directly on gel for electrophoresis.
Areas of use
Classic PCR with "hot" start
Development of PCR products for TA cloning
RT-PCR one- and two-step
Reaction mixture properties
The mix is optimized for the specific performance of HS-Taq DNA polymerase, long-term storage (the storage of BioMaster HS-Taq PCR-Color (2×) at room temperature for 30 days does not affect PCR efficiency), multiple thawing-freezing cycles
The mix contains dyes that do not interfere with the performance of polymerase and components increasing density of sample solution for easy gel loading
Benefits of using
The enzyme with hot-start capability increases specificity, sensitivity and reaction yield
HS-Taq DNA polymerase activation requires not more than 5 min heating
Reduced preparation time
Low contamination risk during the preparation of PCR reaction solution
Standardized conditions of the same-type reactions (reduced pipetting error during mixing PCR components from experiment to experiment)
Easier gel loading. No need in loading buffer due to high density of the mixture
PCR products can be further subjected to TA cloning due to the presence of deoxyadenosine overhangs in amplified DNA
1 year (under proper storage and transportation conditions).
at -20 °С; not more than 50 thawing-freezing cycles.
We carefully prepare our products and are ready to confirm their suitability with a passport.
Each box of our products has a unique lot number in the AA-000-0000 format.
Enter it in the box below and download the certificate in PDF format.