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Features
2× reaction mix containing Hot-Start Taq DNA polymerase for End-Point PCR
Application
Hot-start PCR
High-throughput PCR
Conventional PCR with high reproducibility
Generation of PCR products for TA cloning
RT-PCR (two-step method)
MH010-200
Number of reactions (50 μl)
200
€42
MH010-1020
Number of reactions (50 μl)
1020
€200
MH010-1020
Number of reactions (50 μl)
1020
€1,600
Basic description
BioMaster HS-Taq PCR (2×) kit includes 2× BioMaster HS-Taq PCR reaction mix, 50 mM MgCl2 and sterile water. The kit has been developed for PCR analysis of a large number of samples. BioMaster HS-Taq PCR (2×) reaction mix contains all components (except for DNA template and primers) needed for a PCR reaction: highly processive recombinant HS-Taq DNA polymerase, deoxynucleoside triphosphate mixture, 2× PCR buffer and Mg2+.
The mix is optimized for performing efficient and reproducible hot-start PCR. The master mix contains additional components increasing the half-life and processivity of HS-Taq DNA polymerase by enhancing its stability during PCR. BioMaster HS-Taq PCR (2×) reaction mix is chemically stable, inert and does not interfere with optimal annealing temperature or the parameters of template melting. DNA polymerase included in the kit is inactive at room temperature and requires preheating at 95 °C for 5 min. Additional MgCl2 solution allows easy optimization of the reaction mix for each individual primer/template system. Use of the kit helps saving experimental time and minimizes contamination risk due to reduced number of pipetting steps.
Areas of use
Classic PCR with "hot" start Development of PCR products for TA cloning RT-PCR one- and two-step Reaction mixture properties
The mix is optimized for specific performance of HS-Taq DNA polymerase, long-term storage (the storage of BioMaster HS-Taq PCR (2×) at room temperature for 30 days does not affect PCR efficiency), multiple thawing-freezing cycles
Benefits of using
Hot-start enzyme increases specificity, sensitivity and reaction yield
HS-Taq DNA polymerase activation requires 5 min heating
Reduced preparation time
Low chance of contamination during preparation of PCR solution
Standardized conditions of the same-type reactions (reduced pipetting error during mixing PCR components from experiment to experiment)
PCR products can be further subjected to TA cloning due to the presence of deoxyadenosine overhangs in amplified DNA
Storage
1 year (under proper storage and transportation conditions).
Transportation
at -20 °С; not more than 50 thawing-freezing cycles.
Product passport
We carefully prepare our products and are ready to confirm their suitability with a passport.
Each box of our products has a unique lot number in the AA-000-0000 format.
