Reverse transсriptase M-MuLV–RH

Features
M-MuLV–RH is a genetically modified reverse transcriptase (revertase) from murine leukemia virus (M-MuLV). The enzyme possesses RNA- and DNA-dependent polymerase activity but lacks RNase H activity. Temperature optimum for M-MuLV-RH activity is 42°C (active at temperatures up to 50 °C). The kit also includes 5× RT buffer mix containing all components necessary for revertase performance except for primers and RNA template.
Application
  • First strand cDNA synthesis for RT-PCR and real-time RT-PCR
  • cDNA generation for TA-cloning
  • Generation of labeled cDNA probes for microarrays
  • DNA labeling
  • RNA analysis by primer extension

Basic description

Reverse transcriptase M-MuLV–RH is a genetically modified reverse transcriptase (revertase) from murine leukemia virus (M-MuLV). It differs from wild-type M-MuLV in structure, catalytic features and temperature optimum of activity. The enzyme possesses RNA- and DNA-dependent polymerase activity but lacks RNase H activity. Temperature optimum for M-MuLV-RH activity is 42°C (remains activity at temperatures up to 50 °C). The enzyme is capable of synthesizing first strand cDNA up to 9 kb and incorporating modified bases.
In addition to M-MuLV–RH revertase, the kit also includes 5× RT buffer mix containing all components needed for revertase performance except for RNA template and primers. The buffer is optimized for efficient reaction of reverse transcription from a wide range of RNA templates.

Areas of use

  • CDNA synthesis for cloning
  • Classic PCR with "hot" start
  • DNA tagging

Reaction mixture properties

  • Synthesis of complementary DNA strand from RNA template (RNA-dependent DNA polymerase)
  • Lacks RNase H activity
  • Allows to obtain long cDNA fragments up to 9 kbp
  • Provides high yield of cDNA: the use of 100 U of enzyme per 1 µg of RNA provides not less than 100 ng of first strand cDNA
  • Improved thermostability

Benefits of using

Storage

1 year (under proper storage and transportation conditions).

Transportation

at -20°С; not more than 50 thawing-freezing cycles.

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