Reverse transcriptase RNAscribe RT

Basic description

It allows to synthesize cDNA paragraphs up to 9 kbp. and include modified bases.
It is active within 55 ° C - 60 ° C, it allows to use complex RNA templates and provides specificity of the reaction.
It possesses RNase H activity (convenient for analyzing changes in gene expression).
Provides a high yield of cDNA, the reaction yield is at least 100 ng of the first strand of cDNA at 100 u. RNAscribe RT per 1 mcg of RNA.
Fast reaction rate allows to carry out synthesis in just 15 minutes. The enzyme is resistant to incubation at room temperature during up to 15 days.

Areas of use

  • CDNA synthesis for cloning
  • Classic PCR with "hot" start
  • DNA tagging
  • Preparation of labeled cDNA probes for microarrays

Benefits of using



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