M-MuLV–RH First Strand cDNA Synthesis Kit

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M-MuLV–RH First Strand cDNA Synthesis Kit

M-MuLV–RH First Strand cDNA Synthesis Kit

Features

reaction kit for synthesis of first strand cDNA from a wide range of RNA templates.

Application

First strand cDNA synthesis for RT-PCR and qRT-PCR
cDNA synthesis for cloning
Generation of labeled cDNA probes for microarrays
DNA labeling
RNA analysis by primer extension

Description Benefits Storage and transportation Documentation

Basic description

M-MuLV–RH Reverse Transcription Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. M-MuLV–RH enzyme is a genetically modified reverse transcriptase (revertase) from murine leukemia virus (M-MuLV) that is different from wild-type M-MuLV in structure, catalytic features and temperature optimum of activity. The enzyme possesses RNA- and DNA-dependent polymerase activity but lacks RNase H activity. Temperature optimum for M-MuLV-RH activity is 42°C (the enzyme remains active at temperatures up to 50 °C). The enzyme is able to synthesize first strand cDNA up to 9 kb and incorporate modified bases.

The kit contains two types of buffers based on KCl and (NH4)2SO4 solutions optimized for efficient reaction of reverse transcription from any RNA template, including ones containing regions of particular complexity (highly structured or GC-rich).

Oligo(dT)16 primer and random hexaprimer included in the kit allow more targeted approach for the study of any RNA type or region by reverse transcription. Random hexaprimer non-specifically binds to RNA template and is used for cDNA synthesis from any RNA type contained in total RNA. Oligo(dT)16 primer selectively anneals to the 3'-poly(A)-termini of RNA enabling cDNA synthesis only from mRNA containing a poly(A)-end.

Areas of use

CDNA synthesis for cloning
Classic PCR with "hot" start
DNA tagging
Preparation of labeled cDNA probes for microarrays

Reaction mixture properties

Synthesis of complementary DNA strand from RNA template (RNA-dependent DNA polymerase)
Lacks RNase H activity
Allows to obtain long cDNA fragments up to 9 kb
Provides high yield of cDNA: the use of 100 U of enzyme per 1 µg of RNA provides not less than 100 ng of first strand cDNA
Improved thermostability

Benefits of using

Storage

1 year (under proper storage and transportation conditions).

Transportation

at -20 °С; not more than 50 thawing-freezing cycles.

Product passport

We carefully prepare our products and are ready to confirm their suitability with a passport.

Each box of our products has a unique lot number in the AA-000-0000 format.
Enter it in the box below and download the certificate in PDF format.

General documentation

produkt-_-R01-polnoye-opisaniye_eng.pdf