Plasmid DNA isolation kit for Isolation of plasmid DNA from bacterial cells




50 preps



250 preps

Suspension buffer SB

15 ml

70 ml

Lysis buffer LB


2x35 ml

Neutralization buffer NB

24 ml


Wash buffer WB1


22.5 ml

2x52.5 ml

Wash buffer WB2


6 ml

2x14 ml

Elution buffer EB

2x5 ml

50 ml

Collection tubes and spin columns

50 pcs

250 pcs

Basic description


We are constantly improving the protocol for the kit. Please use the protocol provided with the product.

Updated March 2022


The kit is designed for isolation and purification of plasmid DNA from bacterial cells cultures. The protocol consists of two main operational stages: alkaline lysis of bacterial cells and subsequent binding of nucleic acids from a lysed sample on a silica-gel membrane, followed by washing and the elution of the purified sample.

The isolated DNA can be used for PCR, restriction, sequencing, transformation, transfection, and other applications.

Safety Information

CAUTION! Lysis buffer LB, Neutralization buffer NB, Wash buffer WB1 contain irritant and toxic chemicals. When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. Buffers are toxic in contact with skin and insides, causing burns.

In case of a contact with skin, wash immediately with plenty of water and detergents. Visit a doctor if necessary.

Areas of use

  • Isolation of RNA, DNA and Proteins

Benefits of using


All components of the kit can be stored at room temperature (15-25 °C) for up to 12 months


All components of the kit are shipped at room temperature.

Product passport

We carefully prepare our products and are ready to confirm their suitability with a passport.

Each box of our products has a unique lot number in the AA-000-0000 format.
Enter it in the box below and download the certificate in PDF format.