DNA isolation kit for Isolation of DNA from Reaction Mixtures

Features

Kit components


DR-50

50 preps

DR-250

250 preps

Preparation buffer PB

(concentrate)

12 ml

2x30 ml

Wash buffer WB

(concentrate)

6 ml

2x14ml

Elution buffer EB

2x5 ml

50 ml

Collection tubes and spin columns

50 pcs

250 pcs

Application

Equipment and Reagents to be Supplied by User

Microcentrifuge with rotor for 1.5 ml tubes, speed 10000 rcf

1.5 ml microcentrifuge tubes

Ethanol, 96-100%

Basic description

Important!

We are constantly improving the protocol for the kit. Please use the protocol provided with the product.

Updated January 2022

Description

The kit is designed for isolation and purification of DNA (from 50 to 10000 base pairs) from reaction mixtures. For instance, it is possible to purify DNA from dNTP, enzymes, unbound low molecular weight radioactive and fluorescent labels, etc. The method of DNA isolation is based on the selective binding of nucleic acids from a lysed sample on a silica-gel membrane, followed by washing and the elution of the purified sample. It is possible to isolate up to 10-20 μg of DNA.

The isolated DNA can be used for PCR, transcription, nick-translation, sequencing, and other genetic engineering applications.

The kit does not contain phenol and chaotropic salts like guanidine thiocyanate.

Safety Information

CAUTION! When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. You must follow the rules of general and personal safety when working with the kit.

Areas of use

  • Isolation of RNA, DNA and Proteins

Benefits of using

Storage

All components of the kit can be stored at room temperature (15-25 °C) for up to 12 months.

Transportation

All components of the kit are shipped at room temperature.

Product passport

We carefully prepare our products and are ready to confirm their suitability with a passport.

Each box of our products has a unique lot number in the AA-000-0000 format.
Enter it in the box below and download the certificate in PDF format.