DNA/RNA isolation kit for isolation of DNA/RNA from animal and bacterial cells, swabs, viruses
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Features
Contents
PN-100
100 preps
Lysis buffer LB
70 ml
Precipitation buffer PB
100 ml
Wash buffer WB2
(concentrate)
22 ml
Suspension buffer SB
10 ml
Application
Equipment and Reagents to be Supplied by User
Microcentrifuge with rotor for 1.5 ml tubes, speed 12000 rcf
Water bath or heating block at 65 °C
1.5 ml microcentrifuge tubes
Ethanol, 96-100%
Basic description
Important!
We are constantly improving the protocol for the kit. Please use the protocol provided with the product.
Updated March 2022
Description
The kit is designed for DNA/RNA isolation and purification from animal and bacterial cells cultures, swabs, viruses. Lysis buffer lyses cell membrane releasing nucleic acids. Subsequent steps are precipitation and washing of nucleic acids (DNA/RNA) followed by dissolving of samples.
Note: Lysis buffer contains nucleic acids co-precipitant, therefore using additional co-precipitant reagent using isn’t necessary.
Important! RNase or DNase treatment is required if clean DNA or RNA is needed.
The isolated DNA can be used for PCR, nick-translation, sequencing and other genetic engineering applications.
The isolated RNA can be used for RT-PCR, sequencing and other genetic engineering applications.
Safety Information
CAUTION! When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. You must follow the rules of general and personal safety when working with the kit. Lysis buffer LB contains guanidine thiocyanate solution, which is irritating and toxic. Buffer are toxic in contact with skin and insides, causing burns. In case of a contact with skin, wash immediately with plenty of water and detergents. Visit a doctor if necessary.
CAUTION! Precipitation buffer PB isopropanol, which is irritating and toxic. Don’t work with this buffer near the open fire.
Areas of use
Isolation of RNA, DNA and Proteins
Storage
All components of the kit can be stored at room temperature (15-25 °C) for up to 12 months.
Transportation
All components of the kit are shipped at room temperature.
Product passport
We carefully prepare our products and are ready to confirm their suitability with a passport.
Each box of our products has a unique lot number in the AA-000-0000 format.
