For some time now we have been ordering from you a set of Biomaster LR HS-PCR, the products of which we use to prepare FISH probes. I would like to note some points:
1. The declared length (up to 15 kbw with genomic DNA) really corresponds to what is possible to obtain. This is very important for us, because together with your kit we tested a kit for amplification of long fragments from another company, where the declared length is not remained true even after the introduction of a large number of modifications. Therefore, we decided to stop at your set.
2. To prepare probes for two loci, we use 6 amplification products, 10 kbp each, obtained by the Biomaster LR HS-PCR kit, of which, in most cases, 4 pass immediately on a standard three-step amplification program, and to the rest we apply a number of modifications , which ultimately allows you to achieve the desired result. However, we have not been able to get a product from one locus, but we do not give up hope and are working on it). The products are quite clean, without nonspecific. In order not to be unfounded, in the attached documents the results obtained by us using your set.
3. I would also like to note the presence of detailed recommendations that are spelled out in the product information. In general, we are satisfied with this set.
Thank you and your team!