Frequently asked questions

Category

Choose a product

Can you please tell Is the kit for the isolation of total RNA and microRNA of cells and tissues (catalog number LRU-100-50) suitable for the isolation of ultrapure RNA?

It is difficult to say whether the isolated RNA is ultrapure. The isolated RNA can be used for RT-PCR, Northern blotting and other tasks. We can send a trial kit if needed.

What is ultrapure RNA for? If we knew the problem, it would be easier to answer whether our set is suitable for such purposes.

RNA STABILIZER

As I understand it, the volume is 100 ml, but how long is it enough, is it possible to reuse it? That is, each sample should be soaked in a separate volume, or is it possible to soak everything in one in turn? How long should the sample be kept in solution? Or did I get it all wrong?

For storage in the RNA stabilizer, cut the tissue into small fragments less than 0.5 mm thick. Add the reagent at least 10-fold excess, per 1 mg of tissue - 10 μl of RNA stabilizer, while the reagent should completely cover the sample. Incubate for 24 hours at +4 degrees, after which, for long-term storage, samples can be placed at −20 or −70 degrees.

At +4 degrees, the integrity of the RNA is maintained for at least a month. You can put as many pieces of tissue as you like in one tube with a reagent, the main thing is to observe the above conditions, while it is better to increase the volume of the reagent. Reagent reuse was not tested.

Didn't find the answer?
Ask a question

Enter your data for feedback

A special section of COVID-19 presents ready-made solutions for the isolation and detection of RNA of the SARS-CoV-2019 virus

To learn more