As I understand it, the volume is 100 ml, but how long is it enough, is it possible to reuse it? That is, each sample should be soaked in a separate volume, or is it possible to soak everything in one in turn? How long should the sample be kept in solution? Or did I get it all wrong?
For storage in the RNA stabilizer, cut the tissue into small fragments less than 0.5 mm thick. Add the reagent at least 10-fold excess, per 1 mg of tissue - 10 μl of RNA stabilizer, while the reagent should completely cover the sample. Incubate for 24 hours at +4 degrees, after which, for long-term storage, samples can be placed at −20 or −70 degrees.
At +4 degrees, the integrity of the RNA is maintained for at least a month. You can put as many pieces of tissue as you like in one tube with a reagent, the main thing is to observe the above conditions, while it is better to increase the volume of the reagent. Reagent reuse was not tested.